thiazolyl blue (mtt) Search Results


4 5 dimethylthiazol 2 yl  (Valiant Co Ltd)
96
Valiant Co Ltd 4 5 dimethylthiazol 2 yl
4 5 Dimethylthiazol 2 Yl, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/pmc13035954-370-10-13?v=Valiant+Co+Ltd
Average 96 stars, based on 1 article reviews
4 5 dimethylthiazol 2 yl - by Bioz Stars, 2026-08
96/100 stars
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mtt assay 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide  (Gold Biotechnology Inc)
95
Gold Biotechnology Inc mtt assay 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mtt Assay 3 4 5 Dimethylthiazol 2 Yl 2 5 Diphenyltetrazolium Bromide, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/bio_rxiv__64898__2026__04__06__716844-175-9-13?v=Gold+Biotechnology+Inc
Average 95 stars, based on 1 article reviews
mtt assay 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide - by Bioz Stars, 2026-08
95/100 stars
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thiazolyl blue tetrazolium bromide  (Chem Impex International)
95
Chem Impex International thiazolyl blue tetrazolium bromide
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Thiazolyl Blue Tetrazolium Bromide, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/bio_rxiv__64898__2026__01__27__699126-59-6-12?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
thiazolyl blue tetrazolium bromide - by Bioz Stars, 2026-08
95/100 stars
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mtt solution 0.5% thiazolyl blue  (Carl Roth GmbH)
90
Carl Roth GmbH mtt solution 0.5% thiazolyl blue
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mtt Solution 0.5% Thiazolyl Blue, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/pm40652254-57-0-7?v=Carl+Roth+GmbH
Average 90 stars, based on 1 article reviews
mtt solution 0.5% thiazolyl blue - by Bioz Stars, 2026-08
90/100 stars
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3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (mtt)  (Chemie GmbH)
90
Chemie GmbH 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (mtt)
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
3 (4,5 Dimethylthiazol 2 Yl) 2,5 Diphenyl Tetrazoliumbromide (Mtt), supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/pmc08074366-44-52-67?v=Chemie+GmbH
Average 90 stars, based on 1 article reviews
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (mtt) - by Bioz Stars, 2026-08
90/100 stars
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3-(4,5-dimethyl-2-thizolyl)-2,5-diphenyl-2h-tetrazolium bromide  (Applichem inc)
90
Applichem inc 3-(4,5-dimethyl-2-thizolyl)-2,5-diphenyl-2h-tetrazolium bromide
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
3 (4,5 Dimethyl 2 Thizolyl) 2,5 Diphenyl 2h Tetrazolium Bromide, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/pmc09227539-153-3-7?v=Applichem+inc
Average 90 stars, based on 1 article reviews
3-(4,5-dimethyl-2-thizolyl)-2,5-diphenyl-2h-tetrazolium bromide - by Bioz Stars, 2026-08
90/100 stars
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thiazolyl blue tetrazolium bromide (mtt  (Biomatik)
90
Biomatik thiazolyl blue tetrazolium bromide (mtt
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Thiazolyl Blue Tetrazolium Bromide (Mtt, supplied by Biomatik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/10__1039_slash_D1NR03507J-89-0-14?v=Biomatik
Average 90 stars, based on 1 article reviews
thiazolyl blue tetrazolium bromide (mtt - by Bioz Stars, 2026-08
90/100 stars
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mtt (thiazolyl blue) reagent  (Promega)
90
Promega mtt (thiazolyl blue) reagent
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mtt (Thiazolyl Blue) Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/10__1128_slash_cdli__8__5__972___979__2001-93-3-7?v=Promega
Average 90 stars, based on 1 article reviews
mtt (thiazolyl blue) reagent - by Bioz Stars, 2026-08
90/100 stars
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thiazole blue tetrazole bromide mtt  (KeyGene Inc)
90
KeyGene Inc thiazole blue tetrazole bromide mtt
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Thiazole Blue Tetrazole Bromide Mtt, supplied by KeyGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/pm36949426-90-39-46?v=KeyGene+Inc
Average 90 stars, based on 1 article reviews
thiazole blue tetrazole bromide mtt - by Bioz Stars, 2026-08
90/100 stars
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thiazolyl blue tetrazolium bromide (mtt) biochemica  (PanReac AppliChem)
90
PanReac AppliChem thiazolyl blue tetrazolium bromide (mtt) biochemica
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Thiazolyl Blue Tetrazolium Bromide (Mtt) Biochemica, supplied by PanReac AppliChem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/pmc10970880-122-0-22?v=PanReac+AppliChem
Average 90 stars, based on 1 article reviews
thiazolyl blue tetrazolium bromide (mtt) biochemica - by Bioz Stars, 2026-08
90/100 stars
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mtt (thiazolyl blue tetrazolium bromide), cell culture tested  (HiMedia Laboratories)
90
HiMedia Laboratories mtt (thiazolyl blue tetrazolium bromide), cell culture tested
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mtt (Thiazolyl Blue Tetrazolium Bromide), Cell Culture Tested, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thiazolyl+blue+%28mtt%29/10__5455_slash_jabet__2022__d141-44-4-13?v=HiMedia+Laboratories
Average 90 stars, based on 1 article reviews
mtt (thiazolyl blue tetrazolium bromide), cell culture tested - by Bioz Stars, 2026-08
90/100 stars
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mtt (thiazolyl blue tetrazolium bromide) q105  (PanEco Scientific)
90
PanEco Scientific mtt (thiazolyl blue tetrazolium bromide) q105
( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by <t>MTT</t> <t>assay</t> at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mtt (Thiazolyl Blue Tetrazolium Bromide) Q105, supplied by PanEco Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by MTT assay at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: bioRxiv

Article Title: Integrating Fas-mediated apoptosis with IFNγ signaling to drive tumor regression in mRNA cancer therapeutics

doi: 10.64898/2026.04.06.716844

Figure Lengend Snippet: ( A ) Schematic diagram of IFNγ-FasICD and IFNγ-FasΔ proteins. SP; The IFNγ signal sequence peptide, FasTM; Fas transmembrane domain, FasICD; Fas intracellular domain. A linker (GGGSGGGSGGGS) was added. Numbers indicate amino acid positions. (B) Model of IFNγ-FasICD working mechanism. ( C-E ) HEK293T cells were transfected with IFNγ-FasICD mRNA. (C) IFNγ levels in cell lysates were measured by ELISA at 6 h. (D) The expression of membrane-bound IFNγ was analyzed using flow cytometry. (E) IFNγ levels in culture supernatants were measured at 6 h. (F) MC38 and B16OVA cells were transfected with Luc (luciferase), IFNγ-FasICD, or IFNγ-FasΔ mRNA, cell viability was assessed by MTT assay at 24 h. (G-H) B16OVA cells were transfected with the IFNγ-FasICD mRNA. Caspase-3/7 activity was measured to assess apoptosis (G) and Annexin V + cells were quantified (H) . (I) Expression levels of IFNγ receptor 1 and 2 ( Ifngr1 and Ifngr2 ) were analyzed by semi-quantitative RT-PCR. β-actin ( Actb ) was used as an internal control. (J) Expression of IFNγ downstream target genes, Irf1 and Cd274 , was analyzed by real-time PCR. (K) B16OVA cells were transfected with IFNγ-FasICD mRNA and co-cultured with splenocytes from C57BL/6J mice. STAT1 phosphorylation (Tyr701) in splenocytes as analyzed over time by flow cytometry (left and middle panels), and the percentage of pSTAT1 + cells at the 60-min time point among CD45 + cells is shown (right panel). Data are pooled from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test (C, E, G), ordinary one-way ANOVA followed by Šídák’s multiple comparisons test (J), two-way ANOVA followed by Dunnett’s multiple comparisons test (F), and unpaired t-tests (H, K). Error bars indicate SEM. Note: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: After 24 hours, cell viability was assessed using the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; GoldBio, T-030-1, USA).

Techniques: Sequencing, Transfection, Enzyme-linked Immunosorbent Assay, Expressing, Membrane, Flow Cytometry, Luciferase, MTT Assay, Activity Assay, Quantitative RT-PCR, Control, Real-time Polymerase Chain Reaction, Cell Culture, Phospho-proteomics